RECOMBINANT PLASMID DNA pYAII-39, USED FOR 4TH AND 9TH HUMAN CHROMOSOME MARKING IN METAPHASIC AND INTERPHASIC CELLS

摘要

FIELD: organic chemistry, genetics. ^ SUBSTANCE: invention refers to genetic engineering. Recombinant plasmid DNA pYAll-39 contains: - Pstl-Pstl-DNA vector fragment pUC19 sized 2700 nucleotide pairs, - Pstl- Pstl - alphoid DNA fragment of human lymphocytes high-specific for centromeric areas of 4th and 9th human chromosomes sized 680 nucleotide pairs. The latter is produced from Pstl restriction fragments of alphoid DNA total fraction as chromosomes hybridization in situ under special conditions of high stiffness with standard saline solution with 55% phormamide. Genetic marker is -Ampr - gen of ampicillin-resistance. Invention enables to reliably identify 4th and 9th human chromosomes in norm and pathology, including preimplantation, prenatal and postnatal diagnostics of chromosome anomaly including aneuploidy at various cancer types, as well as for cytogenetic human genome mapping. ^ EFFECT: identification of 4th and 9th human chromosomes in norm and pathology. ^ 1 cl, 4 ex