THROMBIN MUTANT

摘要

A thrombin mutant in which at least serine at position 205 among amino ac ids in the active center of the thrombin B-chain has been substituted with a nother amino acid, and further any one or more of the following substitution s have been introduced: (I) substitution of arginine at position 89 in the B -chain with another amino acid; (II) substitution of threonine at position 6 9 in the B-chain or serine at position 22 in the B-chain with another amino acid; (III) substitution of alanine at position 200 in the B-chain with anot her amino acid; and (IV) substitution of lysine at position 65 in the B-chai n with threonine.