摘要 |
Articles and methods for determining an analyte indicative of a disease condition are provided. In some embodiments, articles and methods described herein can be used for determining a presence, qualitatively or quantitatively, of a component, such as a particular type of cell, in a fluid sample. In one particular embodiment, a low-cost microfluidic system for rapid detection of T cells is provided. The microfluidic system may use immobilized antibodies and adhesion molecules in a channel to capture T cells from a fluid sample such as a small volume of blood. The captured T cells may be labeled with a metal colloid (e.g., gold nanoparticles) using an antibody specific for the T Cell Receptor (TCR), and metallic silver can be catalytically precipitated onto the cells. The number of T cells captured can be counted and may indicate a disease condition of a patient such as severe combined immune deficiency or human immunodeficiency virus. |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE;BUTTE, MANISH, J.;WONG, AMY, P.;SHARPE, ARLENE, H.;WHITESIDES, GEORGE, M. |
发明人 |
BUTTE, MANISH, J.;WONG, AMY, P.;SHARPE, ARLENE, H.;WHITESIDES, GEORGE, M. |