System and method for the production of recombinant glycosylated proteins in a prokaryotic host

摘要

Abstract The present invention relates generally to a system and a method for the production of recombinant N-glycosylated target proteins. In one aspect, the system of the present invention comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.