Lentiviral vectors for gene transfer in quiescent (G0) cells

摘要

Vector particles of the present invention are derived from HIV-2 and further pseudotyped with a G protein from the VSV viral strain. Such vectors provide a means for efficiently transducing cells residing in the G0 phase of the cell cycle at a low MOI value. Thus the vector particles of the present invention can be readily utilized to safely and efficiently transduce quiescent cells including stem cells or precursor cells without the need to activate said cells by cell culture media and/or cytokines or growth factors. Thus, the vector particles of the present invention provide an improved means for applications in the fields of gene therapy, directed immunotherapy or vaccination, as HIV-2 is a human pathogen and, consequently, during treatment with pharmaceutical compositions based on the present vector particles, no additional foreign pathogen is thereby transferred to a human subject.