IDENTIFICATION OF CLUBROOT RESISTANCE GENE USING RAPD MARKER IN CHINES CABBAGE

摘要

A method for identification of a clubroot resistance gene in Chinese cabbage is provided to reduce the identification time by performing identification with RAPD(randomly amplified polymorphic DNA) marker in a laboratory without a resistance examination process in the field, and screen a clubroot-resistant individual rapidly in a relatively small space. A clubroot resistance gene in Chinese cabbage is identified by confirming the genotype of Chinese cabbage clubroot resistance with RAPD marker, and selecting a resistant individual "CR-serona", wherein the resistance gene analysis of "CR-serona" is performed by discerning the resistance gene pattern of Chinese cabbage clubroot by using single spore-derived pathogenic bacteria(Rrace 4, Willinams method) and a group of F2, BC1P1 and BC1P2, and extracting DNAs from resistant and susceptible individuals from 230 individuals of F2 group and developing DNA marker closely associated with the resistance gene by BSA-RAPD(bulked segregant analysis-randomly amplified polymorphic DNA); and the RAPD marker is a DNA fragment amplified specifically by PCR(polymerase chain reaction) of Chinese cabbage genome by using RAPD primer(OPJ05).