A PURE FORM OF RAPAMYCIN AND A PROCESS FOR RECOVERY AND PURIFICATION THEREOF

摘要

The present invention relates to a pure form of rapamycin with a total im purity content less than 1.2% ; a process for recovery and purification of r apamycin comprising steps of (a) treating the fermentation broth, extracts o r solutions containing rapamycin with water immiscible solvent and concentra tion; (b) addition of a water miscible solvent to effect separation of impur ities present; (c) optionally, binding of the solvent containing the product from step (b) to an inert solid, washing the solid with a base and acid, fo llowed by elution; (d) subjecting the elute from step (c) or the solvent con taining the product from step (b) to silica gel chromatography; (e) crystall ization of the product obtained from step (d); (f) subjecting a solution of the product from step (e) to hydrophobic interaction or reversed phase chrom atography; and (g) re-crystallization to afford rapamycin in substantially p ure form.