| 摘要 |
The invention relates to methods and apparatuses for determining factor X a inhibitors, especially heparins and fractionated or low molecular weight h eparins, and direct FXa inhibitors, in blood samples, which are characterize d in that in a first step the blood sample is brought into contact with a de tection reagent which comprises at least one thrombin substrate which consis ts of a peptide residue which can be eliminated by thrombin and which is ami dically linked via the carboxyl end to an electrogenic substance, and with a known amount of factor X reagent and an activator reagent which brings abou t conversion of factor X into factor Xa, subsequently in a second step the a mount or activity of the electrogenic substance which is eliminated from the thrombin substrate through the factor Xa-mediated thrombin activation, and/ or the time course thereof, is determined as measured signal using electroch emical methods, and finally in a third step, by means of this measured signa l, the amount of the factor Xa inhibitor in the sample of the blood to be in vestigated, or a measured quantity correlating therewith, in particular a cl otting time correlating therewith, is ascertained. |
