ANALYSIS OF RECEPTOR BIOLOGY USING LIGAND COATED BEADS

摘要

A method for capturing activated receptor signaling complexes from live c ells, utilizing bead based biology wherein live cells are contacted with lig and coated beads to form bead binding sites and thereby initiating formation of a ligand-receptor complex at said bead binding site; and a process for d istinguishing and confirming non-specifically bound proteins from specifical ly bound receptor complexes by utilization of one or more methods of biochem ical or biophysical analysis, thereby providing, in a preferred embodiment, a utilization of confocal microscopy and proteomic mass spectroscopy.