ENZYMATIC HYDROLYSIS OF STARCH

摘要

Provided herein are methods of increasing the enzymatic rate of hydrolysis of starch substrates. In certain embodiments the method comprises contacting a starch substrate with one or more amylase family enzymes in the presence of greater than 0.001 mM manganese ions. In certain embodiments, the method comprises contacting a starch substrate with one or more glucoamylase and/or ß-amylase enzymes in the presence of greater than 0.001 mM calcium ions (Ca<SUP>++</SUP>). In certain embodiments, the method comprises contacting the starch substrate with a ß- amylase in the presence of greater than 0.001 mM manganese ion, calcium ion, magnesium ion (Mg<SUP>++</SUP>), strontium ion (Sr<SUP>++</SUP>), barium ion (Ba4<SUP>+</SUP>) or any combination of said metal ions. In certain embodiments the method comprises contacting the starch substrate with a glucoamylase in the presence of greater than 0.001 mM manganese ion, calcium ion, lithium ion (Li<SUP>+</SUP>), potassium ion (K<SUP>+</SUP>) or any combination of said metal ions. Also provided herein are compositions and kits for hydrolyzing starch.