| 摘要 |
A method is provided for determining in real time the course of thrombin activity in a sample of clotting blood or plasma as it appears in and disapp ears from the sample which comprises adding a signal substrate to said sampl e, said signal substrate causing a detectable signal related to the amount o f conversion product formed upon reaction by the generated proteolytic activ ity, monitoring the signal development in time in said sample to provide a c urve, and mixing said sample frequently so that clot formation occurs in a d ense manner such that the majority of the sample remains fluid and that cell precipitation is inhibited, wherein said monitoring and mixing steps are re peated and performed in an alternate way.
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