PRODUCTION OF ALPHA-LINOLENIC ACID IN SUNFLOWER

摘要

The following invention relates to the method of transformation of Sunflower with Agrobacterium tumefaciens harboring the recombinant vector comprising the nucleic acid sequence encoding the enzyme, Delta- 15 desaturase required for alpha-linolenic acid biosynthesis, driven by ubiquitin promoter. Further disclosed are the nucleic acid sequences, constructs, vectors comprising the gene encoding for Delta- 15 desaturase enzyme required for the alpha-linolenic acid production in functional combination with the heterologous regulatory sequences and the host cells into which the vectors have been introduced. Further aspects of the invention relates to the selection and regeneration of the putative transformants based on a method of positive selection using Xylose as a selectable marker.