发明名称 |
Method for generating diversity |
摘要 |
The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b) isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.
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申请公布号 |
US7368259(B2) |
申请公布日期 |
2008.05.06 |
申请号 |
US20030733532 |
申请日期 |
2003.12.11 |
申请人 |
MEDICAL RESEARCH COUNCIL |
发明人 |
SALE JULIAN EDWARD;NEUBERGER MICHAEL SAMUEL;CUMBERS SARAH JANE;TAKEDA SHUNICHI |
分类号 |
C12N15/00;C12Q1/04;A01K67/027;C07K16/00;C12N5/08;C12N5/10;C12N9/00;C12N15/01;C12N15/09;C12N15/10;C12N15/63;C12P21/02 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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