| 摘要 |
An improved method of enzyme-link immunosorbent assay (ELISA) is disclosed, which can be adapted to be implemented by an analytic apparatus having a testing platform and a conducting platform, being arranged separate from the testing platform, and comprises steps of: applying a specific agent upon a reaction zone of the testing platform; dripping a testing antibody onto the testing platform for enabling the testing antibody to be bound by the specific antigen; dripping a cleaning fluid onto the testing platform for washing out any excess testing antibody not being bound by the specific antigen; dripping a bio-reagent onto the testing platform whereas the bio-reagent is capable of binding with the specific antigen and the testing antibody; attaching a side of the conducting platform, having conducting circuit formed thereon, to the reaction zone of the testing platform while connecting an extending end of the conducting platform to an evaluation instrument; and dripping an enzyme onto the reaction zone while enabling the evaluation instrument to generate a value of potential representing the reaction result of the enzyme.
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