| 摘要 |
The present invention provides microdevices for electrochemical detection of target agents in a fluid sample. The microdevices each comprise an elongated microstructure having an inlet for introduction of a fluid sample potentially containing a target agent, and an outlet to allow the sample to flow through the region of the microdevice that allows detection of the target agent. This detection is mediated through hybridization of nucleic acids corresponding to a target agent with device-associated nucleic acids and subsequent binding of an electrochemical detection agent to allow the transfer of an electron to or from the electrode. In specific embodiment, the device comprises: a hollow elongated microstructure with a conductive surface on the internal surface; b) insulating polymer that is uniformly distributed on the conductive surface; c) adapter molecule associated with the insulating polymer, and d) a plurality of associated nucleic acids conjugated to the polymer surface in a specific orientation.
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