Optimizing glycan processing in plants

摘要

Disclosed are methods for optimizing glycan processing in plants so that a glycoprotein having complex type bi-antennary glycans and thus containing galactose residues on both arms and which are devoid of (or reduced in) xylose and fucose can be obtained. A plant or plant cell capable of expressing a heterologous glycoprotein that is reduced in xylose and fucose, can be produced by introducing into said plant, or portion thereof, or said plant cell (a) a first expression vector comprising nucleic acid encoding a hybrid enzyme, said hybrid enzyme comprising at least a portion of a transmembrane region of a plant glycosyltransferase and a catalytic region of mammalian beta 1 ,4-galactosyltransferase and (b) a second expression vector comprising nucleic acid encoding a heterologous glycoprotein.