AN IMMUNO CONJUGATE AND PROCESS FOR PREPARATION THEREOF

摘要

Present invention deals with a new immuno-reagent comprising hapten (mercaptopropinic acid derivative of atrazine) -protein-gold for pesticides detection. The conjugate MPAD-protein-gold competes with the analyte of interest for a finite number of binding sites provided by anti-atrazine antibodies coated on nitrocellulose membrane. The newly developed conjugate has a long shelf life with high stability at 40C. The dynamic concentration range for standard atrazine solutions shows a linear inhibition (decrease in intensity of color) between 10 ppb to 1 ppm atrazine in water samples.