Selection procedure by excess ligands

摘要

SELECTION PROCEDURE IMPLEMENTED BY EXCESS LIGANDS comprising a stochastic method based on the probability of the existence of a high number of possible ligands against the same target. The objective of this protocol is to provide libraries with concentrations of ligands compatible with the equilibrium concentrations in solution. This invention provides ligands consisting of oligonucleotides and each of them has a sequence-defined structure capable of recognizing the target used for the selection. The advantage of this method over the other methods is the ability to obtain a large number of specific ligands in only one step and in a few hours. When proteins are used as targets together with combining oligonucleotide libraries, the ligands obtained are of a nanomolar range affinity and are sensitive to the constitution thereof. Moreover, this method allows for the detection of differential targets of complex mixtures, without need of pure substances.