发明名称 |
Yeast cells expressing modified G proteins and methods of use therefor |
摘要 |
The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. The yeast cells of the present invention can be used in screening assays which can be used to screen for modulators of G protein coupled receptors. Specifically, the invention provides novel yeast cells which express a heterologous G protein coupled receptor and mutant and/or chimeric G protein subunit molecules which serve to functionally integrate the heterologous into the pheromone signaling pathway of the yeast cell. The invention also provides for the expression of heterologous G protein coupled receptors which are functionally integrated into the yeast cell membrane using a yeast alpha factor leader sequence. Drug discovery assays using the subject yeast cells are also provided.
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申请公布号 |
US7361498(B2) |
申请公布日期 |
2008.04.22 |
申请号 |
US20040967087 |
申请日期 |
2004.10.15 |
申请人 |
CADUS TECHNOLOGIES, INC. |
发明人 |
FOWLKES DANA M.;BROACH JAMES R.;MANFREDI JOHN P.;PAUL JEREMY I.;TRUEHEART JOSHUA;KLEIN CHRISTINE A.;MURPHY ANDREW J. M. |
分类号 |
C12N1/20;C07H17/00;C07K14/00;C07K14/395;C07K14/47;C12N15/81;G01N33/50 |
主分类号 |
C12N1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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