METHOD FOR PURIFYING POLYPEPTIDES

摘要

A process is provided for selectively separating a polypeptide of interest f rom components of differing in a mixture comprising the steps of: a) passing the mixture through underivatized silica particles such that the polypeptide adheres to the sili ca particles; b) washing the silica particles to remove impurities; and c) eluting the pol ypeptide from the silica particles with a buffer compris ing an alcoholic or polar aprotic solvent and an alkaline earth, an alkali metal, o r an inorganic ammonium salt,