CELL LINE WHICH IS TRANSFECTED WITH VECTORS CONTAINING IDH3 ALPHA PROMOTER AND LUCIFERASE, AND SEARCHING METHOD OF OBESITY CONTROLLING MATERIAL USING THE SAME

摘要

A transformed cell line is provided to identify a chemical material controlling expression of an IDH3A gene at a transcription step and also find out an anti-obese material such as genistein. An expression vector pIDH3A comprises: a kanamycin resistant gene(Kana+) and a neomycin resistant gene(Neo+); a luciferase gene(LUC+); and an IDH3A promoter of SEQ ID : NO. 1 and has a structure depicted in Fig. 2. A cell line PRDC IDH3A transformed by the expression vector is deposited as a deposition no. KCTC 10981BP. A method for searching an obesity controlling material comprises the steps of: (a) treating the cell line with an obesity controlling candidate material; and (b) detecting the fluorescence emitting reaction, wherein the obesity controlling candidate material is treated with at least two concentrations.