The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
申请公布号
WO2008041002(A2)
申请公布日期
2008.04.10
申请号
WO2007GB03798
申请日期
2007.10.08
申请人
SOLEXA LIMITED;RIGATTI, ROBERTO;OST, TOBIAS;FASHENA, SARAH