METHOD AND SYSTEM FOR FED-BATCH CULTIVATION OF HYDROGEN-OXIDIZING BACTERIA

摘要

The method describes the fed-batch cultivation of hydrogen-oxidizing bacteria in an open system where the effluent gas is discharged at atmospheric pressure. The economical use of CO2 is achieved by keeping the carbon dioxide concentration in the effluent gas at very low values. This is achieved by the following means: 1) decreasing the CO2 concentration in the feeding gas lower than evaluated by the stoichiometry of its consumption with respect to H2 and O2, 2) circulating the gas back to the cultivation media and 3) feedback regulation of the feeding rate of the fresh gas mixture to keep the CO2 concentration in the effluent at low level. The method is particularly useful in growing hydrogen oxidizing bacteria on isotopically 13C labeled carbon dioxide.