| 摘要 |
Producing polypeptides in eukaryotic cells, comprising culturing cells expressing the polypeptide on microcarriers under conditions and at a setpoint temperature appropriate for expression of the polypeptide, cooling the culture to a predetermined temperature below the setpoint, sedimenting the microcarriers, and harvesting all or part of the culture medium, is new. Independent claims are also included for the following: (1) cultivation of eukaryotic cells, comprising culturing cells on microcarriers under conditions and at a setpoint temperature appropriate for maintaining the culture, cooling the culture to a predetermined temperature below the setpoint, sedimenting the microcarriers, and harvesting all or part of the culture medium; and (2) harvesting polypeptides produced by eukaryotic cells growing in microcarrier culture, comprising cooling the culture to a predetermined temperature below the setpoint of the cultivation, and sedimenting the microcarriers. |
