<p>Lipolytic enzyme variants with increased specificity for short-chain fatty acids can be designed on the basis of a three-dimensional model of a lipolytic enzyme such as C. antarctica lipase A with a substrate analogue such as a fatty acid. An amino acid residue is selected within 10 Å of the carbon atom corresponding to the desired chain-length specificity, and the selected residue is substituted with a larger residue, or an amino acid insertion is made adjacent to the selected residue.</p>
申请公布号
WO2008040738(A1)
申请公布日期
2008.04.10
申请号
WO2007EP60472
申请日期
2007.10.02
申请人
NOVOZYMES A/S;SVENDSEN, ALLAN;VIND, JESPER;PATKAR, SHAMKANT ANANT;BORCH, KIM
发明人
SVENDSEN, ALLAN;VIND, JESPER;PATKAR, SHAMKANT ANANT;BORCH, KIM