发明名称 |
METHODS OF GENERATION AND USE OF IN VIVO BIOTINYLATED RECOMBINANT ANTIBODIES |
摘要 |
The present invention provides compositions and methods for generating in vivo biotinylated, secreted recombinant polypeptides comprising recombinantly co-expressing in a yeast cell (a) a secreted recombinant polypeptide comprising a biotin accepting site; and (b) a biotin ligase fused to the golgi localization domain so that the biotin ligase is localized to the golgi of the yeast cell where it acts on the biotin-accepting site of the secreted polypeptide as it transits the secretory system.
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申请公布号 |
US2008085539(A1) |
申请公布日期 |
2008.04.10 |
申请号 |
US20070669811 |
申请日期 |
2007.01.31 |
申请人 |
SCHOLLER NATHALIE;GARVIK BARBARA M |
发明人 |
SCHOLLER NATHALIE;GARVIK BARBARA M. |
分类号 |
C12P21/02;C07K14/00;C07K16/00;C12N1/19;C12N15/63 |
主分类号 |
C12P21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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