发明名称 |
Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method |
摘要 |
Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.
|
申请公布号 |
US7354707(B2) |
申请公布日期 |
2008.04.08 |
申请号 |
US20010891517 |
申请日期 |
2001.06.27 |
申请人 |
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCEAND TECHNOLOGY;KANKYO ENGINEERING CO., LTD. |
发明人 |
KURANE RYUICHIRO;KANAGAWA TAKAHIRO;KAMAGATA YOICHI;TORIMURA MASAKI;KURATA SHINYA;YAMADA KAZUTAKA;YOKOMAKU TOYOKAZU |
分类号 |
C12Q1/68;C07H21/00;G06F19/00 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|