发明名称 |
GERÄT DAS DIE FREIGABE VON NUKLEOTIDEN WÄHREND EINER REAKTION STEUERT |
摘要 |
The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction. |
申请公布号 |
DE60225157(D1) |
申请公布日期 |
2008.04.03 |
申请号 |
DE2002625157 |
申请日期 |
2002.09.05 |
申请人 |
INTEL CORP. |
发明人 |
RAO, VALLURI;NEUBAUER, GABI;KIRCH, STEVEN;YAMAKAWA, MINEO;BERLIN, ANDREW |
分类号 |
C12Q1/68;B01L3/00;C07H21/02;C07H21/04;C12M1/00;C12M1/34;C12N15/00;C12N15/09;C12P19/34;G01J3/42;G01J3/427;G01J3/44;G01N21/65;G01N33/53;G01N33/566 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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