Method for detecting small regulatory RNAs

摘要

A method for detecting small oligonucleotides, the method comprising providing a biological isolate containing at least one small oligonucleotide. The biological isolate is contacted with at least one labelled detection oligonucleotide probe and at least one bridge oligonucleotide under conditions such that the at least one small oligonucleotide and the at least one detection oligonucleotide are preferentially added to the bridge oligonucleotide to produce at least one labelled small oligonucleotide. At least one ligating reagent is added to preferentially join the at least one small oligonucleotide and the at least one detection oligonucleotide in a 'splinted' ligation reaction. The at least one labelled small oligonucleotide may then be detected. The small oligonucleotides may include small regulatory RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA).