| 摘要 |
An in vitro human skin test assay protocol is described wherein a colony of epidermal cells forming an island of such cells is produced from an explant of human skin tissue which has been treated with the enzyme Dispase to enable the removal of almost all the epidermal layer. Said island of cells develops from remaining epidermal cells and "autoengineers" itself to form a round multilayer structure. Laboratory tissue culture techniques using the natural cellular interactions between human epidermal and dermal cells of the skin is described. Said protocol can be used to determine whether a radioisotope, element, molecule or compound or any mixture thereof, applied to the skin topically, transdermally, or subcutaneously in solid, gaseous, liquid form or as a cream causes any effect on skin cellular architecture. |
