The present invention relates to methods for engineering VH domains to improve their solubility and stability. The invention provides for the incorporation of defined amino acid substitutions based on 3-D structural information into the V segments of a heavy chain locus, expressing the locus in a non-human mammal and selecting soluble VH domains. Further stabilising or solubilising mutations maybe introduced as a result affinity maturation during B-cell maturation in vivo.
申请公布号
WO2008035216(A2)
申请公布日期
2008.03.27
申请号
WO2007IB03647
申请日期
2007.09.18
申请人
ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAM;GROSVELD, FRANKLIN, GERARDUS;JANSSENS, RICHARD, WILHELM;DRABEK, DUBRAVKA