METHOD FOR EXTRACTING SUBSTANCES WITH ANTINEOPLASIC ACTIVITY FROM THE CEROPEGIA FUSCA PLANT, AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM

摘要

Method for extracting substances with antineoplasic activity from the Ceropegia fusca plant, and pharmaceutical compositions containing them. The method involves macerating the fresh plant with ethanol at 25°C; evaporating the solvent using a vacuum and at a temperature of 40°C; extracting the remaining aqueous extract in countercurrent and at reflux using CH<SUB>2</SUB>CI<SUB>2</SUB> and subsequently n-butanol. The component substances of the extract are purified by means of silica gel column chromatography using the n-hexane:CHCI<SUB>3</SUB>:acetone system in the ratio 1:1:1 as eluents. The extract obtained from Ceropegia fusca has antineoplasic components and can be used in pharmaceutical compositions containing said extract for these purposes.