ESTERASE GENE (EST5S) ENCODING A NEW GROUP ESTERASE ENZYME IDENTIFIED FROM COW RUMEN METAGENOME AND AMINO ACID SEQUENCE ENCODED THEREFROM

摘要

A sequence of an esterase coding gene(est5S) is provided to be isolated from ruminant stomach bacteria, which is not be cultured by a conventional technique. An esterase is provided to be a novel esterase not belonging to a conventional fat hydrolysis group and show significant effect on metal ions and organic solvent, thereby being useful for food industry, environment industry, agricultural industry and medicinal industry. A novel esterase gene hydrolyzing fat includes a sequence described as SEQ ID : NO. 1 derived from ruminant stomach bacteria identified from cow rumen metagenome. An esterase hydrolyzing the fat includes an amino acid sequence described as SEQ ID : NO. 2 derived from the ruminant stomach bacteria identified from cow rumen metagenome.