APIASE PROTEIN CONSTRUCT

摘要

The invention relates to a protein construct for producing soluble proteins. The aim of the invention is to provide a simple and therefore inexpensive method of producing many different kinds of polypeptides in soluble form. For this purpose, a protein construct comprises a fusion protein with a first amino acid sequence segment and a second amino acid sequence segment, the first amino acid sequence segment coding for a secondary amide peptide bond-specific cis/trans isomerase (APIase) and the second amino acid sequence segment coding for any first polypeptide, or else the construct comprises a polypeptide associate, containing a first polypeptide molecule provided with a first tag and a second polypeptide molecule provided with a second tag, the first polypeptide molecule being an APIase and the second polypeptide molecule being any second polypeptide and the first tag specifically binding to the second tag, preferably with a thermodynamic affinity constant of less than 1 µM.