Supplementation of sequence based typing of methicillin-resistant S. aureus (MRSA) by PCR-based grouping of SCCmec-elements; sequencing directly from the multiplex-PCR

摘要

The present invention relates to a spa -sequence based typing of MRSA, which is performed directly on the sample for the multiplex-PCR used for identifying the staphylococcal cassette chromosome mec (SCCmec)-groups. In particular, the method for typing strains or lineages of MRSA, comprises the steps of a) providing a DNA sample comprising DNA of an MRSA strain to be analysed, b) performing a multiplex DNA amplification of said DNA in said sample, wherein at least one sequence of an SCC mec -element and an X-region of a spa- gene are co-amplified, in order to produce a master amplification-mix, c) analysing the amplified SCCmec-element(s) and the X-region(s) of the spa-gene directly from said master amplification-mix, and d) typing said strains or lineages of MRSA based on said analysis.