发明名称 |
Process for the production of proteins by fermentation |
摘要 |
<p>Producing a heterologous protein (I) comprises culturing an Escherichia colistrain on a technical scale in a fermentation medium containing more than 4 mg/l calcium ions or more than 48 mg/l magnesium ions, whereby (I) is secreted into the medium, and separating (I) from the medium. The Escherichia colistrain has a mutation in the lpp gene or its promoter and comprises a (I)-encoding protein linked to a signal sequence encoding a signal peptide.</p> |
申请公布号 |
EP1903105(A1) |
申请公布日期 |
2008.03.26 |
申请号 |
EP20060121090 |
申请日期 |
2006.09.22 |
申请人 |
WACKER CHEMIE AG |
发明人 |
DASSLER, TOBIAS;WICH, GUENTER;SCHMID, GERHARD |
分类号 |
C12N1/20 |
主分类号 |
C12N1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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