Method and Apparatus for Isolating and Purifying Nucleic Acid by Single Bead

摘要

Provided is a method of isolating and purifying nucleic acid from cells or viruses, the method comprising: mixing a sample containing cells or viruses with a solution comprising beads dispersed in a binding buffer to bind the cells or viruses with the beads; separating the beads having the cells or viruses bound thereto and then washing the beads with the buffer; disrupting the cells or viruses bound to the beads to bind nucleic acid released from the cells or viruses with the beads; washing the beads having nucleic acid bound thereto with the buffer; and adding a nucleic acid eluting buffer to the beads to elute nucleic acid bound to the beads, wherein a compound represented by Formula 1 is bound to the surface of the bead.