Method of renewing a biological environment containing cells in suspension

摘要

Replacing a biological medium in which cells are suspended comprises passing the medium via a cell-retaining filter to a discharge outlet and then passing fresh medium through the filter in the opposite direction. Independent claims are also included for: (1) transfer system for carrying out the above process, comprising a first tube connected at one end to a vessel containing the suspension and at the other end to a vessel containing the fresh medium, a filter mounted in the first tube, a second tube connected to the first tube between the filter and the vessel containing the fresh medium, and a collection vessel connected to the second tube; (2) cell culture assembly comprising a transfer system as above in which the vessel containing the suspension is a culture vessel.