发明名称 |
Discrimination method of target base in DNA, and allele specific primer used in the method of the same |
摘要 |
An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3' end base is designed to be the base corresponding to SNP; the second base from the 3' end to be T or G; the third base from the 3' end to be any one of A, T or C; and the base sequence of from the fourth from the 3' end to the 5' end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3' side to a desired base.
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申请公布号 |
US7348150(B1) |
申请公布日期 |
2008.03.25 |
申请号 |
US20060633587 |
申请日期 |
2006.12.05 |
申请人 |
MATSUSHITA ELECTRIC INDUSTRIAL CO., LTD. |
发明人 |
YAKU HIDENOBU;OKA HIROAKI;YUKIMASA TETSUO |
分类号 |
C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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