METHOD FOR DETERMINATION OF INHIBITORY ACTIVITY ON DNA METHYLATION

摘要

Disclosed is a method for detecting an inhibitor substance which inhibits the methylation of DNA rapidly and in a simple manner without the need of using a sample in a large quantity and which can be used for the screening for a DNA methylation inhibitor. The method comprises the steps of: mixing a test DNA having a methylation site therein and having a fluorescent label attached thereto, a substance to be tested and a DNA-methylating enzyme; reacting the test DNA with a methylation-sensitive active substance capable of cleaving an unmethylated methylation site selectively; measuring a fluorescence intensity of the test DNA after the reaction with the methylation-sensitive restriction enzyme; and determining whether or not the test DNA is cleaved with the methylation-sensitive active substance based on the fluorescence intensity, wherein it is determined whether or not the substance to be tested is a inhibitor substance capable of inhibiting the methylation of the test DNA depending on whether or not the test DNA is cleaved.