METHOD, POLYNUCLEOTIDE PROBE, DNA CHIP AND KIT FOR IDENTIFYING MUTATION OF HUMAN MITOCHONDRIAL DNA

摘要

A method for identifying mutation of human mitochondrial DNA is provided to inspect rapidly and accurately a plurality of mutations on the human mitochondrial DNA and haplotype of the mitochondrial DNA in one slide through one experiment. A method for identifying mutation of human mitochondrial DNA comprises the steps of: extracting DNA from the blood, cells or tissues of human; performing PCR(polymerase chain reaction) of the extracted DNA to obtain PCR products containing at least one of HV1(hypervariable region) and HV2 regions contained in the control region of human mitochondrial DNA; hybridizing the PCR products with a polynucleotide probe containing at least one DNA sequence of SEQ ID NO:5 to SEQ ID NO:90 or its complementary nucleotide sequence; and determining the mutation of human mitochondrial DNA by confirming the hybridization.