A PREPARATION OF AN ARTIFICIAL TRANSCRIPTION FACTOR COMPRISING ZINC FINGER PROTEIN AND TRANSCRIPTION FACTOR OF PROKARYOTE, AND AN USE THEREOF

摘要

An artificial transcription factor polypeptide is provided to control the activity of various prokaryotes by using a catabolite controlling protein of the prokaryotes as an effect domain, and induce various transformed E. coli(s) showing characteristics desired by a user through activation or inhibition of genes regardless of the action of the transcription factor inside of the E. coli by introducing the same thereinto, thereby obtaining the transformed E. coli with improved heat-resistance, low temperature resistance, high salinity resistance and growth speed. An artificial transcription factor polypeptide for activating or inhibiting gene expression artificially comprises 1 to 3 zinc finger domain(s) and a transcription factor of prokaryote, wherein the zinc finger domain is selected from the group consisting of zinc finger domains coded by nucleic acid sequences of SEQ ID : NOs. 13(Z1), 15(Z2), 17(Z3), 19(Z4), 21(Z5), 23(Z6), 25(Z7), 27(Z8), 29(Z9), 31(Z10), 33(Z11), 35(Z12), 37(Z13), 39(Z14), 41(Z15), 43(Z16), 45(Z17), 47(Z18), 49(Z19), 51(Z20), 53(Z21), 55(Z22), 57(Z23), 59(Z24), 61(Z25) and 63(Z26), and the transcription factor is selected from the group consisting of a wild type CRP(catabolite regulatory protein)(CRPW, residue 1-209) coded by SEQ ID : NO. 1, a CRP Del 137(residue 137-90) coded by SEQ ID : NO. 3 and a CRP Del 180(residue 1-180) coded by SEQ ID : NO. 5. A transformed E. coli is prepared by introducing the artificial transcription factor into the E. coli and has the heat-resistance and the resistance against the growth speed improvement, low temperature or high salt.