| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a new D-amino acid oxidase, to provide a method for producing the same, and to provide a method for producing an L-amino acid, 2-oxo acid or cyclic imine using the same. <P>SOLUTION: A new D-amino acid oxidase gene isolated from Candida intermedia is provided. A recombinant plasmid containing the above gene is provided. A transformant transferred with the above gene is provided. The method for producing the D-amino acid oxidase is also provided, comprising culturing the Candida intermedia or the above transformant and retrieving the D-amino acid oxidase. Further, the method for producing an L-amino acid, 2-oxo acid or cyclic imine by making the D-amino acid oxidase act on a racemic amino acid is provided. In addition, another version of the method for producing an L-amino acid is provided, comprising subjecting a racemic amino acid to the existence of the D-amino acid oxidase together with an amino acid dehydrogenase and an enzyme having the ability to regenerate a coenzyme. <P>COPYRIGHT: (C)2008,JPO&INPIT |
