CHARGE PERTURBATION DETECTION SYSTEM FOR DNA AND OTHER MOLECULES
摘要
Methods and apparatus for direct detection of chemical reactions are provided. In a preferred embodiment, electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The target molecule is preferably DNA. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time.
申请公布号
WO2007008246(A3)
申请公布日期
2008.03.06
申请号
WO2005US40558
申请日期
2005.11.10
申请人
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY;POURMAND, NADER;KARHANEK, MILOSLAV;DAVIS, RONALD, W.
发明人
POURMAND, NADER;KARHANEK, MILOSLAV;DAVIS, RONALD, W.