Image analysis methods and apparatus are used for distinguishing live and dead cells. The methods may involve segmenting an image to identify the region(s) occupied by one or more cells and determining the presence of a particular live-dead indicator feature within the region(s). In certain embodiments, the indicator feature is a cytoskeletal component such as tubulin. In certain embodiments, the methods may involve determining the value of an indicator expression that is based on cellular components such as DNA and/or cellular protein. Prior to producing an image for analysis, cells may be treated with a marker that highlights the live-dead indicator in the image.
申请公布号
WO2007095359(A3)
申请公布日期
2008.03.06
申请号
WO2007US04125
申请日期
2007.02.13
申请人
CYTOKINETICS, INC.;KUTSYY, VADIM;FAN, JINHONG;VAISBERG, EUGENI, A.