CHEMOENZYMATIC PROCESS FOR THE PREPARATION OF IMINOCYCLITOLS

摘要

The present invention discloses a chemoenzymatic process for the preparation of an iminocyclitol corresponding to formula (I), (II), (III) or (IV), wherein: R<SUP>1</SUP> and R<SUP>2</SUP> are the same or different, and independently selected from the group consisting of: H, OH, hydroxymethyl, methyl, ethyl, butyl, pentyl, hexyl, octyl, isopropyl, isobutyl, 2-methylbutyl, and benzyl; R<SUP>3</SUP> is selected from the group consisting of: H, hydroxymethyl, hydroxyethyl, ethyl, butyl, pentyl, hexyl, octyl, dodecyl, isobutyl, isopropyl, isopentyl, 2- methylbutyl, benzyl, and phenylethyl; n: O or 1; the process comprising: (i) an aldol addition catalyzed by a D-fructose-6-phosphate aldolase enzyme (FSA) and an acceptor aminoaldehyde; and (ii) an intramolecular reductive amination of the addition adduct obtained in step (i) with H<SUB>2</SUB>, in the presence of a metallic catalyst, optionally being carried out said step (ii) in the presence of an aldehyde of formula R<SUP>3</SUP>-CHO, wherein R<SUP>3</SUP> is as defined above.