METHODS FOR PRODUCING TRANSGENIC CORN PLANTS IN A SINGLE CONTAINER
摘要
<p>A method for producing a transformable and embryogenic corn cell culture comprising: a) obtaining a Type II callus from corn embryos; b) initiating a cell suspension culture from cells of the callus; and c) culturing the cell suspension for about 5 days to 30 days in a liquid medium to produce the cell culture, wherein the cell culture is transferred to fresh growth media every 2-3 days; and wherein undesirable cell types are removed from the cell culture during transfer to fresh growth media.</p>
申请公布号
CA2884022(A1)
申请公布日期
2008.03.06
申请号
CA20072884022
申请日期
2007.08.31
申请人
MONSANTO TECHNOLOGY LLC
发明人
AKULA, ANISHA;DUNCAN, DAVID R.;LOWE, BRENDA;MANN, MICHAEL T.;PETERSEN, WILLIAM L.;ROUT, JYOTI R.;SONGSTAD, DAVID D.;WILKS, JOEL B.;ZHANG, WANGGEN