发明名称 |
COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS |
摘要 |
Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of al,3-fucosyltransferase (FucT) and ß 1 ,2-xylosyltransferase (XyIT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a "humanized" N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the GO glycoform, are also provided. |
申请公布号 |
WO2007084922(A3) |
申请公布日期 |
2008.03.06 |
申请号 |
WO2007US60642 |
申请日期 |
2007.01.17 |
申请人 |
BIOLEX, INC.;DICKEY, LYNN, F.;COX, KEVIN, M.;PEELE, CHARLES, G. |
发明人 |
DICKEY, LYNN, F.;COX, KEVIN, M.;PEELE, CHARLES, G. |
分类号 |
C12N15/82;A01H5/00;C07K14/47;C07K16/00;C12P21/00 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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