发明名称 |
In vivo gene transfer |
摘要 |
The present invention is directed to an in vivo method of transferring DNA from a donor cell to a recipient cell. In a specific embodiment, the method comprises a highly efficient process of bacterial mating. In a preferred embodiment, selection for a recombinant plasmid against a parent host plasmid and donor plasmid is based on recircularization of the host plasmid by its recombination with a gene of interest such that it is now no longer cleaved by a restriction enzyme expressed in the recipient cell.
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申请公布号 |
US7338800(B2) |
申请公布日期 |
2008.03.04 |
申请号 |
US20030345523 |
申请日期 |
2003.01.16 |
申请人 |
BAYLOR COLLEGE OF MEDICINE |
发明人 |
ELLEDGE STEPHEN J.;LI MAMIE Z. |
分类号 |
C12N15/74;C12N1/20;C12N1/21;C12N15/00;C12N15/70 |
主分类号 |
C12N15/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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