| 摘要 |
FIELD: organic chemistry, chemical technology. ^ SUBSTANCE: invention proposes a method for purifying macrolide compounds. Invention describes a method for isolation of macrolide in a purified state that involves the following steps: (a) treatment of an unpurified macrolide or crude macrolide with a solvent not mixing with water; (b) not obligatory concentrating the mixture; (c) treatment with gaseous ammonia to precipitate impurities; (d) separation of impurities; (e) not obligatory concentration of macrolide-containing phase; (f) carrying out chromatography on silica gel column in not obligatory reversed-phase, or with preliminary treatment with silver and elution of macrolide; (g) preparing macrolide in the essential purified form; (h) not obligatory repeat of steps (f) and (g) for preparing macrolide in the essential purified form. Macrolide is represented preferably as tacrolimus, immunomycin or syrolimus. Proposed method provides preparing macrolides of high purity degree by simplified technology. ^ EFFECT: improved isolating and purifying method. ^ 8 cl, 2 ex |
